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ECRG4: a new potential target in precision medicine
Xin Qin, Ping Zhang
《医学前沿(英文)》 2019年 第13卷 第5期 页码 540-546 doi: 10.1007/s11684-018-0637-9
关键词: ECRG4 tumor suppressor gene sentinel molecule precision medicine cell senescence epithelium homeostasis
null
《医学前沿(英文)》 2014年 第8卷 第2期 页码 227-235 doi: 10.1007/s11684-014-0327-1
Accelerated senescence is important because this process is involved in tumor suppression and has been induced by many chemotherapeutic agents. The platinum-based chemotherapeutic agent cisplatin displays a wide range of antitumor activities. However, the molecular mechanism of cisplatin-induced accelerated senescence in hepatocellular carcinoma (HCC) remains unclear. In the present study, the growth inhibitory effect of cisplatin on HepG2 and SMMC-7721 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cellular senescence was then assessed by β-galactosidase assay. Senescence-related factors, including p53, p21, and p16, were evaluated by quantitative reverse transcription-polymerase chain reaction. Reactive oxygen species (ROS) was analyzed by flow cytometry. Our results revealed that cisplatin reduced the proliferation of HepG2 and SMMC-7721 cells in a dose- and time-dependent manner. Senescent phenotype observed in cisplatin-treated hepatoma cells was dependent on p53 and p21 activation but not on p16 activation. Furthermore, cisplatin-induced accelerated senescence depended on intracellular ROS generation. The ROS scavenger N-acetyl-L-cysteine also significantly suppressed the cisplatin-induced senescence of HepG2 and SMMC-7721 cells. In conclusion, our results revealed a functional link between intracellular ROS generation and cisplatin-induced accelerated senescence, and this link may be used as a potential target of HCC.
关键词: reactive oxygen species senescence cisplatin hepatocellular carcinoma
null
《医学前沿(英文)》 2014年 第8卷 第1期 页码 106-112 doi: 10.1007/s11684-014-0307-5
Cervical carcinoma is associated with high propensity for local invasion and lymph node metastasis. However, the molecular alterations that drive progression and metastasis of cervical cancer remain unclear. Cellular senescence has been proposed as the mechanism that protects an organism against cancer progression and metastasis. In addition, Twist, a basic helix-loop-helix transcription factor, has been suggested as an oncogene because it is overexpressed in many types of human cancer. This gene also exhibits a positive function in regulating invasion and metastasis. In this study, Twist was strongly and positively expressed in normal tissue, squamous cell carcinoma (SCC) IA--IIA, and SCC IIB--IIIB (4.3%, 44%, and 88.9%, respectively). The strong positive expressions of the senescence marker CBX3 were 39.1%, 32%, and 15.6%, respectively. The strong positive expressions of Twist in the SCC groups with or without lymph node metastasis were 80.8% and 50%. For CBX3, such expressions were 7.7% and 29.5%, respectively. Results also showed that the expression of Twist was inversely correlated with that of CBX3. Moreover, the knockdown of Twist with target siRNA in SiHa triggered the induction of the chromatin marker of the cellular senescence CBX3 and senescence-associated β-galactosidase activity. Our results suggested that the expression of Twist increased during the progression and metastasis of cervical cancer. Furthermore, Twist-induced senescence bypass is important in this process.
关键词: cervical cancer senescence Twist CBX3 lymph node metastasis
Biqiong Fu, Jie Yang, Jia Chen, Lirong Lin, Kehong Chen, Weiwei Zhang, Jianguo Zhang, Yani He
《医学前沿(英文)》 2019年 第13卷 第2期 页码 267-276 doi: 10.1007/s11684-017-0586-8
关键词: Shenkang injection senescence renal tubular epithelial cells diabetic nephropathy
Taking advantage of drug resistance, a new approach in the war on cancer
null
《医学前沿(英文)》 2018年 第12卷 第4期 页码 490-495 doi: 10.1007/s11684-018-0647-7
Identification of the driver mutations in cancer has resulted in the development of a new category of molecularly targeted anti-cancer drugs. However, as was the case with conventional chemotherapies, the effectiveness of these drugs is limited by the emergence of drug-resistant variants. While most cancer therapies are given in combinations that are designed to avoid drug resistance, we discuss here therapeutic approaches that take advantage of the changes in cancer cells that arise upon development of drug resistance. This approach is based on notion that drug resistance comes at a fitness cost to the cancer cell that can be exploited for therapeutic benefit. We discuss the development of sequential drug therapies in which the first therapy is not given with curative intent, but to induce a major new sensitivity that can be targeted with a second drug that selectively targets the acquired vulnerability. This concept of collateral sensitivity has hitherto not been used on a large scale in the clinic and holds great promise for future cancer therapy.
关键词: cancer drug resistance genetic screens senescence targeted therapy
Qi RUI, Qin LU, Dayong WANG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 76-90 doi: 10.1007/s11684-009-0002-0
关键词:
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
《化学科学与工程前沿(英文)》 2016年 第10卷 第1期 页码 90-98 doi: 10.1007/s11705-015-1550-2
关键词: microfluidic chip co-culture cell-cell interactions review
Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in
《医学前沿(英文)》 页码 939-956 doi: 10.1007/s11684-023-0987-9
关键词: mononuclear diploid cardiomyocytes cell–cell communication cardiac fibroblast single-cell RNA sequencing cardiac regeneration
Deubiquitinases as pivotal regulators of T cell functions
null
《医学前沿(英文)》 2018年 第12卷 第4期 页码 451-462 doi: 10.1007/s11684-018-0651-y
T cells efficiently respond to foreign antigens to mediate immune responses against infections but are tolerant to self-tissues. Defect in T cell activation is associated with severe immune deficiencies, whereas aberrant T cell activation contributes to the pathogenesis of diverse autoimmune and inflammatory diseases. An emerging mechanism that regulates T cell activation and tolerance is ubiquitination, a reversible process of protein modification that is counter-regulated by ubiquitinating enzymes and deubiquitinases (DUBs). DUBs are isopeptidases that cleave polyubiquitin chains and remove ubiquitin from target proteins, thereby controlling the magnitude and duration of ubiquitin signaling. It is now well recognized that DUBs are crucial regulators of T cell responses and serve as potential therapeutic targets for manipulating immune responses in the treatment of immunological disorders and cancer. This review will discuss the recent progresses regarding the functions of DUBs in T cells.
关键词: deubiquitinase ubiquitination T cell activation T cell differentiation T cell tolerance
Cell surface protein engineering for high-performance whole-cell catalysts
Hajime Nakatani,Katsutoshi Hori
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 46-57 doi: 10.1007/s11705-017-1609-3
关键词: cell surface engineering surface display whole-cell catalysts bioprocess
Stem cell niches and endogenous electric fields in tissue repair
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 40-44 doi: 10.1007/s11684-011-0108-z
Adult stem cells are responsible for homeostasis and repair of many tissues. Endogenous adult stem cells reside in certain regions of organs, known as the stem cell niche, which is recognized to have an important role in regulating tissue maintenance and repair. In wound healing and tissue repair, stem cells are mobilized and recruited to the site of wound, and participate in the repair process. Many regulatory factors are involved in the stem cell-based repair process, including stem cell niches and endogenous wound electric fields, which are present at wound tissues and proved to be important in guiding wound healing. Here we briefly review the role of stem cell niches and endogenous electric fields in tissue repair, and hypothesize that endogenous electric fields become part of stem cell niche in the wound site.
CAR T-cell immunotherapy: a powerful weapon for fighting hematological B-cell malignancies
《医学前沿(英文)》 2021年 第15卷 第6期 页码 783-804 doi: 10.1007/s11684-021-0904-z
Aldolase B attenuates clear cell renal cell carcinoma progression by inhibiting CtBP2
《医学前沿(英文)》 2023年 第17卷 第3期 页码 503-517 doi: 10.1007/s11684-022-0947-9
The unregulated commercialization of stem cell treatments: a global perspective
Douglas Sipp
《医学前沿(英文)》 2011年 第5卷 第4期 页码 348-355 doi: 10.1007/s11684-011-0150-x
关键词: stem cell tourism medical ethics stem cell policy and regulation alternative medicine
Fine-tuning cell organelle dynamics during mitosis by small GTPases
《医学前沿(英文)》 2022年 第16卷 第3期 页码 339-357 doi: 10.1007/s11684-022-0926-1
标题 作者 时间 类型 操作
Reactive oxygen species generation is essential for cisplatin-induced accelerated senescence in hepatocellular
null
期刊论文
Correlation of Twist upregulation and senescence bypass during the progression and metastasis of cervical
null
期刊论文
Preventive effect of Shenkang injection against high glucose-induced senescence of renal tubular cells
Biqiong Fu, Jie Yang, Jia Chen, Lirong Lin, Kehong Chen, Weiwei Zhang, Jianguo Zhang, Yani He
期刊论文
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
期刊论文
Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in
期刊论文
Cell surface protein engineering for high-performance whole-cell catalysts
Hajime Nakatani,Katsutoshi Hori
期刊论文